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After selection of 3 g/ml puromycin(Beyotime, Cat# ST551), TPC-1 and IHH4 cells were harvested for western blotting to assess RRM2 knockdown efficiency

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With respect to the gut microbiota, treatment with urolithin A increased the abundance of commensal bacteria ( Lactobacillus , Lachnospiraceae ) and suppressed the proliferation of pathogenic bacteria ( Clostridia , Candidatus ) compared with vehicle-treated, sleep-deprived controls

It is not clear whether the -elimination reactions with the busulfan S -conjugates on balance are detoxification or bioactivation events